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The present study explored the anti-urolithic activity of parmotrema perlatum extract. It was carried out with the help of non
polar to polar solvent by using soxhlet extraction method. This extraction was performed until the solvent become colorless
and solvents were evaporated to get active compound. By using this extract preliminary phytochemical screening was
performed to identify the phytoconstituent present in the extract are alkaloids, flavonoids, essential oil, glycosides, phenol,
saponin, terpenoids, tannins, usnic acid etc. There after extract was evaluated for anti-urolithic activity using titrimetric method
(semi-permeable egg membrane). In which cystone used as standard and calcium oxalate crystal was prepared in laboratory.
Blank, standard, samples are packed into semi-permeable membrane of egg along with calcium oxalate crystal and then placed
incubator 37°Cfor 2 hours, for about 7-8 hours. Removed the contents of semi-permeable membrane from each group into a
test tube. Added 2 ml of 1 N sulphuric acid and titrated with 0.9494 N KMnO4 till a light pink color end point obtained.1ml of
0.9494 N KMnO4 equivalent to 0.1898mg of Calcium. The amount of undissolved calcium oxalate is subtracted from the total
quantity used in the experiment in the beginning, to know how much quantity of calcium oxalate actually test substance(s)
could dissolve. Finally, percentage dissolution was calculated which helps to determine the antiurolithic activity of
parmotrema perlatum. In addition, molecular docking studies were performed to confirm the antiurolithiasis activity
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